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barcode oligonucleotides  (New England Biolabs)


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    Structured Review

    New England Biolabs barcode oligonucleotides
    Barcode Oligonucleotides, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4487 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcoding+primers/Nuclease-free+Water/pm41776501-44-26-36
    Average 99 stars, based on 4487 article reviews
    barcode oligonucleotides - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
    Article Snippet: The concentration was measured using a Qubit dsDNA High Sensitivity assay kit (Invitrogen). .. A 10 ng aliquot of input or immunoprecipitated DNA in three replicates per condition were used in library preparation for dual index Illumina paired-end sequencing ( Para et al. , 2018 ) with the following modifications: (i) using NEBNext Multiplex Oligos (New England Biolabs) for adaptor ligation and barcoding primers, following the manufacturer’s protocols; (ii) using AMPure XP beads (Beckman Coulter) for size selection; and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (New England Biolabs). .. Libraries were pooled and sequenced (paired-end 2×150 bp) on an Illumina NovaSeq 6000 at the Purdue Genomics Core Facility.

    Sequencing:

    Article Title: Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
    Article Snippet: The concentration was measured using a Qubit dsDNA High Sensitivity assay kit (Invitrogen). .. A 10 ng aliquot of input or immunoprecipitated DNA in three replicates per condition were used in library preparation for dual index Illumina paired-end sequencing ( Para et al. , 2018 ) with the following modifications: (i) using NEBNext Multiplex Oligos (New England Biolabs) for adaptor ligation and barcoding primers, following the manufacturer’s protocols; (ii) using AMPure XP beads (Beckman Coulter) for size selection; and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (New England Biolabs). .. Libraries were pooled and sequenced (paired-end 2×150 bp) on an Illumina NovaSeq 6000 at the Purdue Genomics Core Facility.

    Multiplex Assay:

    Article Title: Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
    Article Snippet: The concentration was measured using a Qubit dsDNA High Sensitivity assay kit (Invitrogen). .. A 10 ng aliquot of input or immunoprecipitated DNA in three replicates per condition were used in library preparation for dual index Illumina paired-end sequencing ( Para et al. , 2018 ) with the following modifications: (i) using NEBNext Multiplex Oligos (New England Biolabs) for adaptor ligation and barcoding primers, following the manufacturer’s protocols; (ii) using AMPure XP beads (Beckman Coulter) for size selection; and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (New England Biolabs). .. Libraries were pooled and sequenced (paired-end 2×150 bp) on an Illumina NovaSeq 6000 at the Purdue Genomics Core Facility.

    Article Title: Chromatin-remodeling factor SMARCD2 regulates transcriptional networks controlling differentiation of neutrophil granulocytes
    Article Snippet: Isolated nuclei were treated with Tn 5 transposase from the Nextera DNA Library Preparation kit (Illumina, FC-140-1089) for 30 min at 300 r.p.m. in a Thermomixer. .. Transposed DNA was purified with the Qiagen MinElute Reaction Cleanup kit (Qiagen, 28204) and amplified with Illumina Tn 5-compatible barcoding primers ( Supplementary Table 3 ; NEBNext Multiplex Oligos for Illumina, New England BioLabs). ..

    Ligation:

    Article Title: Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
    Article Snippet: The concentration was measured using a Qubit dsDNA High Sensitivity assay kit (Invitrogen). .. A 10 ng aliquot of input or immunoprecipitated DNA in three replicates per condition were used in library preparation for dual index Illumina paired-end sequencing ( Para et al. , 2018 ) with the following modifications: (i) using NEBNext Multiplex Oligos (New England Biolabs) for adaptor ligation and barcoding primers, following the manufacturer’s protocols; (ii) using AMPure XP beads (Beckman Coulter) for size selection; and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (New England Biolabs). .. Libraries were pooled and sequenced (paired-end 2×150 bp) on an Illumina NovaSeq 6000 at the Purdue Genomics Core Facility.

    Size Selection:

    Article Title: Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
    Article Snippet: The concentration was measured using a Qubit dsDNA High Sensitivity assay kit (Invitrogen). .. A 10 ng aliquot of input or immunoprecipitated DNA in three replicates per condition were used in library preparation for dual index Illumina paired-end sequencing ( Para et al. , 2018 ) with the following modifications: (i) using NEBNext Multiplex Oligos (New England Biolabs) for adaptor ligation and barcoding primers, following the manufacturer’s protocols; (ii) using AMPure XP beads (Beckman Coulter) for size selection; and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (New England Biolabs). .. Libraries were pooled and sequenced (paired-end 2×150 bp) on an Illumina NovaSeq 6000 at the Purdue Genomics Core Facility.

    Polymerase Chain Reaction:

    Article Title: Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
    Article Snippet: The concentration was measured using a Qubit dsDNA High Sensitivity assay kit (Invitrogen). .. A 10 ng aliquot of input or immunoprecipitated DNA in three replicates per condition were used in library preparation for dual index Illumina paired-end sequencing ( Para et al. , 2018 ) with the following modifications: (i) using NEBNext Multiplex Oligos (New England Biolabs) for adaptor ligation and barcoding primers, following the manufacturer’s protocols; (ii) using AMPure XP beads (Beckman Coulter) for size selection; and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (New England Biolabs). .. Libraries were pooled and sequenced (paired-end 2×150 bp) on an Illumina NovaSeq 6000 at the Purdue Genomics Core Facility.

    Amplification:

    Article Title: Dynamic histone acetylation in floral volatile synthesis and emission in petunia flowers
    Article Snippet: The concentration was measured using a Qubit dsDNA High Sensitivity assay kit (Invitrogen). .. A 10 ng aliquot of input or immunoprecipitated DNA in three replicates per condition were used in library preparation for dual index Illumina paired-end sequencing ( Para et al. , 2018 ) with the following modifications: (i) using NEBNext Multiplex Oligos (New England Biolabs) for adaptor ligation and barcoding primers, following the manufacturer’s protocols; (ii) using AMPure XP beads (Beckman Coulter) for size selection; and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (New England Biolabs). .. Libraries were pooled and sequenced (paired-end 2×150 bp) on an Illumina NovaSeq 6000 at the Purdue Genomics Core Facility.

    Article Title: Chromatin-remodeling factor SMARCD2 regulates transcriptional networks controlling differentiation of neutrophil granulocytes
    Article Snippet: Isolated nuclei were treated with Tn 5 transposase from the Nextera DNA Library Preparation kit (Illumina, FC-140-1089) for 30 min at 300 r.p.m. in a Thermomixer. .. Transposed DNA was purified with the Qiagen MinElute Reaction Cleanup kit (Qiagen, 28204) and amplified with Illumina Tn 5-compatible barcoding primers ( Supplementary Table 3 ; NEBNext Multiplex Oligos for Illumina, New England BioLabs). ..

    Purification:

    Article Title: Chromatin-remodeling factor SMARCD2 regulates transcriptional networks controlling differentiation of neutrophil granulocytes
    Article Snippet: Isolated nuclei were treated with Tn 5 transposase from the Nextera DNA Library Preparation kit (Illumina, FC-140-1089) for 30 min at 300 r.p.m. in a Thermomixer. .. Transposed DNA was purified with the Qiagen MinElute Reaction Cleanup kit (Qiagen, 28204) and amplified with Illumina Tn 5-compatible barcoding primers ( Supplementary Table 3 ; NEBNext Multiplex Oligos for Illumina, New England BioLabs). ..

    other:

    Article Title: Chromatin-remodeling factor SMARCD2 regulates transcriptional networks controlling differentiation of neutrophil granulocytes
    Article Snippet: Count data were generated from mapped reads using feature-Counts 57 on Ensembl gene models (GRCm38.74).



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